"No item in international law allows the US to misuse its judicial procedures to meddle in the domestic affairs of other countries," Mousavi said on Friday.
The Iranian foreign ministry spokesman said the recent American accusations sought to “lay the groundwork for a coup d’état, regime change or an assault against a sovereign country".
On Thursday, Washington announced that it has “indicted” President Maduro and several top aides for "narco-terrorism".
The US State Department offered a reward of $15 million for information that could lead to the arrest or conviction of the senior Venezuelan figures.
Mousavi said that Washington’s “international application of its domestic laws” against Venezuela threatens the sovereignty of the United Nations and the international community.
He added that international inaction and silence regarding the matter assisted the US’ “illegal behavior”.
“We urge all members of the international community to take a united stance defending multilateralism and to loudly say ‘no’ to the US’ aggressive unilateral actions,” he added.
Venezuelan officials have also firmly accused Washington’s recent accusations.
Speaking during a televised speech from the presidential palace in Caracas late Thursday, Maduro rejected the "spurious and false" US accusations.
He said the claims were part of a plan organized by the US and Colombia to force him out of power.
"You are loathsome, Donald Trump!" Maduro said while being flanked by several officials also indicted by the US State Department.
Maduro, Venezuela’s president since 2013, has been the target of a fierce overthrow campaign by Washington over the past years.
Washington’s latest provocations against Caracas come as wide-spanning US sanctions have been targeting the country as Venezuela seeks to contain the coronavirus pandemic.